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. Author manuscript; available in PMC: 2010 May 8.
Published in final edited form as: J Mol Biol. 2009 Mar 14;388(3):415–430. doi: 10.1016/j.jmb.2009.03.023

Figure 4.

Figure 4

Transcription activation of inaA by variants of Rob and the effects of inducers DIP and DEC. Strain RA4468 containing an inaA-lacZ fusion and pBAD18-SoxS-Rob or variants of pBAD18-Rob were grown to A600 ~0.2 at which time they were treated with the following: 0.02% arabinose only (light grey bars); 0.02% arabinose with 3 mM DIP (grey bars); and 0.02% arabinose with 8 mM DEC (black bars). Cultures were harvested after treatment for 1 hr and β-galactosidase specific activity was determined as described in Materials and Methods. A, Transcription activation of the inaA-lacZ fusion by wild type Rob, SoxS-Rob, and Rob mutants. The β-galactosidase specific activity of wild type Rob was set to 100% and the other values are expressed relative to it. B, Effects of the inducers DIP and DEC on activation of transcription of the inaA-lacZ fusion. The β-galactosidase specific activity of each strain grown in the absence of an inducer (light grey bars) was set to 100% and the respective values for the cultures treated with DIP (grey bars) and DEC (black bars) are expressed relative to the uninduced values. The values for R40A induced by DIP (960%) and DEC (780%) have been truncated for reasons of scale and space.