Table 1.

Effects of DIP and DEC on colony size and determination of the half-life of wild type and variant Rob proteins

			Addition to lactose tetrazolium plates containing 0.2% or 2% arabinose
			No treatment		DIP (1 mM)		DEC (3 mM)
Rob Allele	Representative Western Blot	Half-life	0.2%	2%	0.2%	2%	0.2%	2%
None			normal	normal	normal	normal	normal	normal
Wild type		>20 hr	normal	normal	SMALL	NO GROWTH	SMALL	NO GROWTH
RobR40A		>20 hr	normal	normal	normal	normal	normal	normal
Rob107		<1 min	normal	normal	normal	normal	normal	normal
Rob249		20 min	normal	SMALL	normal	SMALL	normal	SMALL
SoxS-Rob		>20 hr	normal	normal	NO GROWTH	NO GROWTH	NO GROWTH	NO GROWTH
SoxS		2 min	normal	normal	normal	normal	normal	normal

Overnight cultures of strain RA4468 [pBAD18-Rob] carrying different rob alleles were diluted10⁻⁶ and 0.1 ml plated on lactose tetrazolium plates with 0.2% or 2% arabinose along with DIP (1 mM), or DEC (3 mM). Growth phenotypes were determined after incubating the plates at 37°C for 15 hr. In separate experiments, the half-life of the respective proteins was determined by western blotting. The half-life of native SoxS²⁷ was determined previously and the value is included here.