Skip to main content
. Author manuscript; available in PMC: 2009 Aug 17.
Published in final edited form as: Langmuir. 2007 May 9;23(12):6827–6834. doi: 10.1021/la700328r

Table 1.

Probe and Target Sequences

DNA sequencea Number of bases description
probe 5′-HS–(CH2)6–GCAGTATCTTCTATTTCTCCACACTGC–MB-3′ 27 probe modified with MB
ST-25 5′-GTG GAG AAA TAG AAG AT-3′ 17 complementary target with 17 bases
ST-25-3M1 5′-GTG GTG AATTAG ATG AT-3′ 17 three separated mismatches
ST-25-3M2 5′-GTG GAG TTTTAG AAG AT-3′ 17 three contiguous mismatches
FC-22 5′-GCAGTGTG GAG AAATAG AAG AT-3′ 22 complementary target with 22 bases
FC-27 5′-GCAGTGTG GAG AAA TAG AAG AT ACTGC-3′ 27 complementary target with 27 bases
ML-28 5′-GCGTTTTTCGC GTG GAG AAA TAG AAG AT-3′ 28 target complementary with 17 bases and
an 11-base tail forming a loop
ML-38 5′-GCGTTTTTCGC GCAGT GTG GAG AAA TAG AAG AT ACTGC-3′ 38 target complementary with 27 bases and
an 11-base tail forming a loop
a

Underlined bases are those different from the normal target so they indicate mismatches and elongation of the target. Bases in italic type are designed to form a structured loop.