Table 3.
Signal Suppression (%) Obtained with Longer and Bulkier Targets.
Data target |
ST-25 | FC-22 | FC-27 | ML-28 | ML-38 |
---|---|---|---|---|---|
binding schemea |
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high densityb |
71 ± 1 | 83 ± 1 | 88 ± 1 | 72 ± 1 | 85 ± 2c |
medium density |
31 ± 2 | 67 ± 7 | 84 ± 3 | 46 ± 3 | 97 ± 3 |
low density |
42 ± 3 | 71 ± 4 | 76 ± 3 | 57 ± 4 | 78 ± 4 |
See Table 1 for sequences.
Percent values of signal suppression as a result of target hybridization are the average and standard deviation of measurements performed with four independent sensors.
After the 5 h equilibration time employed here, the signal suppression is not complete. All other measurements reflect equilibrated sensors.