Table 4.
Summary of E-DNA Sensor Performance
| sensor | probe DNA concentration used during sensor fabrication (µ M) |
probe density (molecules/cm2) |
mean probe-to- probe separation (nm) |
ACV ip (A) |
signal suppression with ST-25 (%) |
detection limita (nM) |
selectivity ratiob |
average regenerationc (%) |
|---|---|---|---|---|---|---|---|---|
| high density | 5 | 2.1 × 1012 | 6.3 | 7.3 × 10−7 | 71 ± 1 | 10 | 1.18 | 98 |
| medium density | 0.1 | 6.6 × 1011 | 12.6 | 2.9 × 10−7 | 31 ± 2 | 10 | 1.19 | 97 |
| low density | 0.005 | 3.9 × 1010 | 50.9 | 1.6 × 10−8 | 42 ± 3 | 1.23 | 88 |
a
Detection limit given is the concentration of ST-25 producing 10% signal suppression.
b
Ratio between the signal suppression obtained with the complementary target (ST-25) and a mismatched target (ST-25-3M1).
c
Signal recovery obtained after 10 measurement/wash cycles.