Fig. 2.

Only little changes in lipid composition and organization after barrier disruption with acetone. A HPTLC of lipid extracts from untreated mouse epidermis (lane 1), acetone-treated epidermis (lane 2) and lipids extracted by the acetone swab (lane 3) are depicted in panela. The amount of lipids applied per lane, represent a skin surface of 10 mm². Legend on the left indicates the R_f-values of different compounds. SE/WE sterol esters/wax esters, TG triglycerides, CHOL cholesterol, FFA free fatty acids, CER ceramides, acyl-GSL acyl-glycosphingolipids, PE phosphoethanolamine. The scattered intensity (arbitrary units) is plotted as function of scattering vector (q) in panelb. SC sheets of two donors (upper two and lower two patterns) before (untreated) and after acetone treatment were directly mounted into the X-ray beam. The roman numerals indicate the various orders of the LPP: first order (I) located at q = 0.46/nm (d = 13.7 nm), second order (II) at q = 0.94/nm (d = 6.7 nm) and third order (III) at q = 1.42/nm (d = 4.4 nm)