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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Breast Cancer Res Treat. 2008 Sep 19;117(2):243–251. doi: 10.1007/s10549-008-0186-z

Figure 3.

Figure 3

Up-regulation of CXCR4 by estrogen is partly responsible for enhanced migration of MCF7-HER2 cells. (A) Cells were treated with either vehicle or E2 (10nM) for 72 h and were grown in serum free medium for 16 h before plating them on the upper chambers of transwell chambers (Corning Inc.). Cells plated on the upper chambers were allowed to migrate towards 10% FBS in the lower chamber for 24 h. (B) Western blot showing the level of CXCR4 protein in the cells under identical treatment conditions. (C) Cells were treated as mentioned in (A) and were allowed to migrate for 24h towards 10% FBS in the lower chamber in the absence or presence of AMD 3100 (1.25µM), a CXCR4 specific inhibitor. (D) Representative photographs of the lower surface of membranes showing the migrated E2 treated cells in absence or presence of AMD 3100. Cells were counted in four microscopic fields per membrane, in duplicate for each condition and repeated three times, with similar results. Values in panels A and C are mean ± SD.