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. Author manuscript; available in PMC: 2010 Sep 4.
Published in final edited form as: J Mol Biol. 2009 Jul 4;391(5):820–832. doi: 10.1016/j.jmb.2009.06.078

Fig. 2. Alternate model nucleic acid substrates serve as cofactors for CSB dependent ATP hydrolysis in the presence of Mg2+ (top) or Ca2+ (bottom).

Fig. 2

CSB ATPase assays were performed as described in Experimental Procedures. Average relative values (to pUC19 reaction under each metal condition) are plotted with standard deviations of at least 3 independent experiments. Substrate depictions are displayed underneath each graph: from left to right pUC19, 19T, 34G/34C, 54FE/54C, 54FE/54C/18D, and 54FE/54C/18R. Solid lines represent DNA and dashed lines represent RNA. (see Table 1 for oligonucleotide details).