Fig. 5. CSB is unable to use ATP hydrolysis for dsDNA translocation.
Representative gel of triplex displacement assay with yRad54 or CSB in the presence of 4 mM Mg2+. Triplex displacement by increasing concentrations of yRad54 (180 fmol to 1.8 pmol) is shown as a control (left). Reactions with increasing CSB (60 fmol to 1.8 pmol) were performed as described in Experimental Procedures. % triplex strand displacement is indicated below each lane.
