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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Cytometry B Clin Cytom. 2009 Sep;76(5):295–314. doi: 10.1002/cyto.b.20480

Figure 3. Reduced Spillover Using More Excitation Lines.

Figure 3

Signal resolution for CD3 versus CD4 is much improved by excitation of 3 conjugated antibodies with three discreet light sources (left, 405nm, 532nm, 638nm for CD3-Pacific Blue, CD8-PE and CD4-APC) compared to one (right, 405nm for CD3-Pacific Blue, CD4-Amcyan and CD8-Pacific Orange).