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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Cytometry B Clin Cytom. 2009 Sep;76(5):334–344. doi: 10.1002/cyto.b.20473

Figure 2.

Figure 2

Analysis of XIAP expression within PBMCs and LCLs generated from patients with XIAP deficiency and normal controls. Figure A and B show western blot analysis of PBMC lysates of patients 1–4, probed with clone 28 anti-XIAP antibody (A) or clone 2F1 (B). Gels were loaded with 30 mcg of protein. Figures C and D depict LCL lysates from patients 1–5 using the clone 28 antibody. Figure C shows 30 mcg of protein loaded into the gel. Figure D shows the same samples, but with 5 mcg of protein loaded for control and patient 1, and 90mcg of protein loaded for patients 2–5. β-actin serves as a loading control. Figures E and F illustrate flow cytometric detection of XIAP in LCLs generated from patients 1–5, with 2 controls shown for comparison, using the clone 2F1 (E) or clone 48 (F) anti-XIAP antibodies. Debris was excluded from analysis by creating a viable LCL gate based on the appearance of forward and right angle light scatter. Filled histograms represent XIAP staining, while open histograms represent isotype control antibody. Percentages of cells positive for XIAP and the geometric mean channel fluorescence (gMCF) are given within the histogram boxes.