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Flow cytometric detection of XIAP in peripheral whole blood granulocytes, monocytes, CD4+ T cells, CD8+ T cells, NK cells, and B cells, as described in the methods section, using the clone 48 antibody. Gating strategy is as shown. Filled histograms represent XIAP staining, while open histograms represent control antibody. Percentages of cells positive for XIAP and the geometric mean channel fluorescence (gMCF) are given within the histogram boxes.
