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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Mol Cancer Ther. 2009 Aug 11;8(8):2412–2423. doi: 10.1158/1535-7163.MCT-09-0186

Figure 1.

Figure 1

Mig-7 specific antibody decreases HEC1A endometrial carcinoma cell chemoinvasion. (a) Overexpression of amino terminus FLAG-tagged Mig-7 significantly increased Matrigel invasion of HT29 cells compared to vector alone stably transfected cells. Bars represent SEM. Results are after 24 hours of invasion performed in quadruplicate. (b) Mig-7 affinity-purified antibody, but not IgG rabbit isotype antibody, significantly decreased chemoinvasion of HEC1A cells into Matrigel toward HGF (p<0.01). Ab = antibody, + HGF = bottom wells contained 20 ng/ml HGF. Results shown are after 72 hours of invasion and are representative of three independent experiments performed in triplicate. Bars represent SEM. (c) Representative flow cytometry histograms of control normal rabbit IgG and Mig-7 antibody-treated HEC1A cells stained with YO-PRO-1 and propidium iodide (PI). YO-PRO-1 single positive cells (lower oval) are apoptotic, and double positive cells (upper oval) are dead. Mean percentages of three samples are shown. (d) Representative flow cytometry histograms of control IgG and Mig-7 antibody-treated HEC1A cells stained with PI. Cells in G2/M phase are indicated on histograms. Mean percentages of three samples are shown. Flow cytometry experiments were conducted twice with similar results.