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. Author manuscript; available in PMC: 2010 Sep 29.
Published in final edited form as: Neuroscience. 2009 May 29;163(1):222–232. doi: 10.1016/j.neuroscience.2009.05.057

Figure 1. Construction and expression of CD4-AChR subunit loop chimeras.

Figure 1

(A) Schematic showing construction of the chimeric proteins consisting of CD4 extracellular and transmembrane domains fused to the major intracellular loop of the AChR subunits. (B) Alignment of the amino acid sequences for each of the subunits shows little sequence similarity in the intracellular loop region (* denotes identical residues; : & . denote conserved and semi-conserved substitutions, respectively). Sequences in the black boxes show the predicted α-helices. (C) CD4-subunit loop constructs expressed in COS cells were immunoprecipitated and immunoblotted with anti-CD4 antibody. All CD4-subunit loop chimeras were expressed at similar levels. CD4-β loop was also detected by mAb124 that recognizes an epitope in the β loop, and CD4-δ by mAb88b that recognizes the δ loop.