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. Author manuscript; available in PMC: 2010 Aug 15.
Published in final edited form as: Virology. 2009 Jun 23;391(1):57–63. doi: 10.1016/j.virol.2009.05.036

Figure 3.

Figure 3

N-cadherin and filamentous actin expression of HPV16 E6 and/or E7 and early region (ER) in primary human keratinocytes (HFKs). N-Cad expression (upper panels) in a panel of passage and donor matched human foreskin keratinocyte (HFK) populations expressing HPV16 E6 and/or E7 oncoproteins or the HPV16 entire early transcription unit (ER), or transfected with empty vector (C) was assessed by immunofluorescence microscopy. The specificity of the antibody was assessed by transfection of primary human foreskin fibroblasts (HFFs) with N-cadherin siRNA (N-Cad si) or scrambled control siRNA (Ctrl si) followed by immunofluorescence analysis at 48 hours after siRNA transfection. Filamentous actin (lower panels) was visualized by fluorescence microscopy of cells stained with rhodamine-conjugated phalloidin.