Fig. 1.

Generation of wild-type and mutant Hsp72 expressing L929.3 cells with equivalent protein levels. a Schematic representation of Hsp72 and the mutant constructs. b Base vector MSCV-IRES-EGFP (BV), wild-type (WT), and mutant Hsp72 expressing cells were sorted with a set gate for similar EGFP profiles reflecting similar copy numbers of the mutant proteins. c, d EGFP sorted L929.3 cells were immunoblotted with the antibodies, C92, N15 and K20. β-Tubulin was used as a loading control for all western blots. A representative sample blot is shown. Antibody specificity: N15: human Hsp72, C92: human/mouse Hsp72, K20: human/mouse Hsp72/73