Fig. 3.

Effects of recombinant adenovirus directed overexpression of prohibitin in cardiomyocyte on cellular apoptosis induced by oxidative stress. Cardiomyocytes were infected with adenovirus with PHB-coding (Ad-PHB) or non-coding constructs (Ad) at a MOI of 35 pfu/cell for 2 h and maintained in culture media for 24 h. Cardiomyocytes were then cultured in fetal bovine serum-free media for 12 h and treated with 200 μM H₂O₂ for 12 h. Cardiomyocyte apoptosis was analyzed with flow cytometry. The percentage of apoptotic cells was determined according to cells containing subdiploid DNA content. The bar graphs represented the mean ± SEM of results from six replicate experiments. *p < 0.05 as compared with control. ^#p < 0.05 as compared with Ad + H₂O₂