Fig. 4.

Effect of overexpressed prohibitin on H⁺-ATPase synthesis activity and mitochondrial membrane potential in cardiomyocytes injured by oxidative stress. Mitochondria were isolated from four groups of cardiomyocytes including without 200 μM H₂O₂ intervention and adenovirus transfection group (control), 200 μM H₂O₂ intervention group (H₂₂), adenovirus with non-coding tranfection plus 200 μM H₂O₂ intervention group (Ad + H₂₂), and adenovirus with PHB-coding tranfection plus 200 μM H₂O₂ intervention group (Ad-PHB + H₂₂). a mitochondrial H⁺-ATPase synthesis activity was measured by fluorescence bioluminescence and calculated according to the intensity of emitted light of determined content of ATP in the parallel standard test and described as micromole per minute milligram protein. b The cardiomyocyte mitochondrial membrane potential in vitro was measured by flow cytometry. The Rh123 was used to detect changes in the MMP and cellular mean fluorescence intensity was analyzed using Cell Quest software programs. The bar graph represents the mean ± SEM of results from six replicate experiments. *p < 0.05 as compared with control. ^#p < 0.05 as compared with Ad + H₂O₂