Fig. 5.

Hsp70 associates with the DNA repairing molecules, PARP-1 and XRCC1. HeLa or HeLa–siRNA–Hsp70 whole cell extracts, from control or heat-shocked (90 min at 43.5 Hsp70 associates with the DNA repairing molecules, PARP-1 and XRCC1. HeLa or HeLa–siRNA–Hsp70 whole cell extracts, from control or heat-shocked (90 min at 43.5°C and 90 min recovery at 37°C) cells (2 × 10⁷ cells/sample), were prepared in RIPA buffer. Subsequently, control or heat-shocked (90 min at 43.5°C and 90 min recovery at 37°C) cell extracts were subjected to immunoprecipitation with anti-Hsp70 (a, c), anti-PARP-1 (a), or anti-XRCC1 (b). The immunoprecipitations were analyzed with SDS-PAGE and Western blotting using specific antibodies against PARP-1, Hsp70, or XRCC1 to track bindings between PARP-1 (A2, B1, C1), Hsp70 (A1, B2, C3), and XRCC1 (B3, C2) proteins. CE cell extract, IP immunoprecipitation, B beads