Fig. 2.
Effect of Foxa1 overexpression or deficiency on A549 cell apoptosis induced by H2O2. a A549 cells were transfected with pcDNA3.1-Foxa1 and the expression levels of Foxa1 were assessed by Western blotting. Ctrl A549 cells treated with lipofectamine only, Neo vector control group, pcDNA3.1-Foxa1 Foxa1 overexpression group. b Effect of Foxa1 overexpression on H2O2-induced apoptosis of A549 cells. Ctrl A549 cells not treated with H2O2. Results were expressed as mean ± SEM of three duplicate experiments. Cells were treated with H2O2 (0.5 mM) for indicated times. *Statistically significant difference between the Neo group and controls, P < 0.05; #statistically significant difference between the pcDNA3.1-Foxa1 group and the controls, P < 0.05; &statistically significant difference from the Neo group at 24 h, P < 0.05. c A549 cells were transiently transfected with Foxa1 morpholino antisense oligonucleotides. Expression of Foxa1 was assessed by RT-PCR and Western blotting in order to identify basal Foxa1 inhibition. d Effect of Foxa1 deficiency on apoptosis of A549 cells induced by H2O2 (0.5 mM for 24 h). Ctrl A549 cells treated with lipofectamine only, Inv A549 cells transiently transfected with random Foxa1 oligonucleotides, AS A549 cells transiently transfected with Foxa1 morpholino antisense oligonucleotides. *P < 0.05