Fig. 5.

Role of Foxa1 in the regulation of transcription activity of bcl2 promoter in type II pneumocytes. a Type II pneumocytes were transiently co-transfected with an expression plasmid of full-length Foxa1 (500 ng) and a reporter driven by bcl2 promoter (500 ng). The luciferase activity was detected using the Dual Luciferase Reporter System. All transfections were performed at least three times in triplicate. Cells were treated with H₂O₂ (0.5 mM) for 24 h. *Statistically significant difference versus the vector control group (Neo), P < 0.05. ^#Statistically significant difference from the relevant group without H₂O₂ stimulation, P < 0.05. b Transient co-transfection studies were performed in type II pneumocytes using full-length Foxa1 and a reporter driven by each of the truncate bcl2 promoter (500 ng). *Statistically significant difference from the bcl2 −1000/+10 group, P < 0.05. ^#Statistically significant difference from the relevant group without H₂O₂ stimulation, P < 0.05