Fig. 1.

Western blot detection of Hsp27 and Hsp70 in zebrafish embryos. a Developmental time course of Hsp27 expression. Numbers are time of sample harvest in hours postfertilization (hpf). b Concentration effects of scrambled (scr) and antisense hsp27 (hsp27i) PMO injection on Hsp27 expression. Numbers represent injection concentration in millimolar. A sample from uninjected embryos (uninj) is also shown. c Effects of 0.15-mM hsp27i PMO injection on Hsp27 expression in embryos of varying ages. The expression of sarcomeric (sarc) actin is shown for comparison. d Effects of 0.1 mM hsp27i PMO injection on expression of constitutive Hsc70 and inducible Hsp70 in control (CN) and heat-shocked (HS) embryos. Samples were harvested at 36 hpf. All lanes were loaded with equal amounts (30 μg) of total embryo protein