Fig. 6.

Hsp27 immunolocalization in unstressed adult zebrafish tissues. Hsp27 was detected throughout the heart myocardium (Hrt) and skeletal muscle, especially in slow-twitch muscle and at the boundary between external slow and internal fast-twitch muscle (arrow, Ske). Hsp27 was detected within the smooth muscle surrounding the intestinal tract (arrow, Int) and in the lining of the brain tissues (arrow, Br). Hsp27 was also detected at the margin of seminiferous tubules in the testis (Test). Right-hand panels are secondary antibody-only controls of each tissue type. Scale bars are 50 μm. A Western blot probed for Hsp70 and Hsp27 expression in white, or fast-twitch, muscle (wm), red, or slow-twitch, muscle (rm), brain, heart, lens, testis, and gut tissues is shown for comparison