Skip to main content
. Author manuscript; available in PMC: 2010 Feb 27.
Published in final edited form as: Circ Res. 2009 Jan 15;104(4):476–487. doi: 10.1161/CIRCRESAHA.108.185363

Fig. 1. Expression and distribution of miR-221 and miR-222 in balloon-injured rat carotid arteries.

Fig. 1

(A). The time course changes of miR-221 and miR-222 expression determined by qRT-PCR. Note: n=6; *P<0.05 compared with uninjured control. (B). Representative Masson's trichrome staining. (C) Negative control (no SM α-actin antibody, no miRNA probe) for In situ hybridization and immunofluorescence. (D) Scrambled probe control 1 (no SM α-actin antibody, but had scrambled miRNA probe). (E) Scrambled probe control 2 (had SM α-actin antibody and scrambled miRNA probe). (F) In situ hybridization of miR-221 (dot green color), immunofluorescence of smooth muscle cell marker SM α–actin (red color) and cell nuclear staining by DAPI (blue color). (G) In situ hybridization of miR-222 (dot green color), immunofluorescence of smooth muscle cell marker SM α–actin (red color) and cell nuclear staining by DAPI (blue color). Note: Autofluorescence in the elastic laminae is demonstrated as green color, but is not dot green (C–G).