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Comparison of the minimum inhibitory concentration of ampicillin for growth of E. coli TP112 and TP132 strains containing plasmids encoding the various BLIP mutants versus the Ki value for inhibition of TEM-1 β-lactamase by the purified BLIP derivatives.
| Ampicillin MIC (μg/ml) | |||
|---|---|---|---|
| BLIP mutant | E. coli TP112 | E. coli TP132 | Ki (nM) |
| No BLIP | 256 | >256 | - |
| Wt BLIP | 1.5 | 4 | 0.5 |
| E31Q | 1.5 | 6 | 1.4 |
| D49S | 2 | 32 | 16 |
| E73Y | 1.5 | 12 | 3.7 |
| W112R | 1.5 | 12 | 3.9 |
| F142G | 1.5 | 16 | 12 |
