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. Author manuscript; available in PMC: 2010 Jul 17.
Published in final edited form as: Circ Res. 2009 Jun 18;105(2):158–166. doi: 10.1161/CIRCRESAHA.109.197517

Figure 6. KLF5 is the critical target gene of miR-145 that is responsible for miR-145-mediated effect on VSMC phenotypic modulation.

Figure 6

(A). KLF5 expression was increased in VSMCs after PDGF (20 ng/ml)-stimulation. (B). pmiR-145, but not pDNR-CMV, or unrelated pmiR-31 inhibited luciferase activity. In the mutated control group, the inhibitory effect of pmiR-145 on luciferase activity was abrogated. n=5; *P<0.05 compared with vehicle control. (C). Representative western blots of KLF5 in miR-145 modulated VSMCs. (D). 2'OMe-miR-145 increased, whereas pre-miR-145 decreased KLF5 expression in cultured VSMCs. n=6; *P<0.05 compared with oligo control. (E).The effects of KLF5 on the expression of myocardin and SM α–actin. n=5; * P<0.05 compared with siRNA control (si-control) and, # P<0.05 compared with Ad-GFP. (F). The effect of miR-145 on the expression of myocardin. n=6; *P<0.05 compared with oligo control. (G). The effects of pre-miR-145 on the expression of SM α–actin in VSMCs with or without KLF5 depletion. Note: n=6; *P<0.05 compared with oligo control in siRNA control (si-control)-treated groups. #P<0.05 compared with oligo control in KLF5 siRNA-treated groups.