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. Author manuscript; available in PMC: 2009 Aug 18.
Published in final edited form as: Nat Protoc. 2006;1(6):2733–2741. doi: 10.1038/nprot.2006.229

Figure 3. Determination of affinity constants from changes in the intrinsic CD of the polypeptide backbone as a function of increasing ligand concentration.

Figure 3

0.4 ml of chicken muscle Troponin C (TnC), 0.1 mg/ml, in 100 mM KCl, 50 mM Tris-HCl, pH 7.5, 0.9 mM EGTA, 0.9 mM nitriloacetic acid, pH 7.5 at 25 °C, in a 0.2 ml cuvette was titrated with Ca2+.