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Minimal requirements for cardiac gene activation in mouse embryos. a, Expression of Actc in embryos transfected with pairwise combinations of transcription factors+Baf60c. Only Gata4+Baf60c induced ectopic Actc (red arrowheads). b, Expression of Nkx2-5; Gata4+Baf60c induced ectopic Nkx2-5 (arrowheads). c, Percentage of Actc-positive and beating embryos for each combination. d, Specificity of cardiogenic factors; percentage of embryos in which Actc was induced is shown.
