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. 2009 Aug 10;186(3):393–407. doi: 10.1083/jcb.200904105

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© 2009 Yamakoshi et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — Induction of human p16^Ink4a gene expression during the onset of cellular senescence in cultured MEFs. (A and B) Primary MEFs derived from the p16-luc mice were rendered senescent by either serial passage (A) or oncogenic Ras expression (retrovirus infection; B). The levels of endogenous p16^Ink4a expression and luciferase activity were measured. Representative data of three independent experiments are shown. As confirmation of senescence, representative photographs of the cells stained for SA β-gal activity are shown. β-Actin was used as a loading control. The means ± SD of three independent experiments are shown. WB, Western blot.