Figure 7.

FA adhesion dynamics of TLN1, EGFR, and CAV2. Time-lapse videos (Videos 1–8) of HeLa cells transfected with TLN1, EGFR, CAV2, or Risc-free (RF) siRNA were used to create temporal ratio images (a) and for autocorrelation analysis (b). For each siRNA, the ratio between two time points, 10 min apart, is presented in color code: new pixels are blue, pixels that disappeared are red, and unchanged pixels are represented by variable colors, depending on the specific local intensity ratio. Yellow indicates identical intensities in the two time points. Note the high predominance of yellow pixels in TLN1-transfected cells, and note the high proportion of red and blue pixels in EGFR-transfected cells. For each siRNA, four different videos were used to perform autocorrelation analysis. Autocorrelation is calculated by comparing the adhesions at each time point with those at time zero. All points are plotted, as well as the single exponential decay fitting curve. The extracted decay time is: RF = 37.81 ± 2.07 min, TLN1 = 94.25 ± 12.71 min, EGFR = 24.45 ± 1.32 min, and CAV2 = 37.50 ± 0.89 min. The differences between RF, TLN1, and EGFR are highly significant (P < 0.0001). The knockdown of the three siRNAs is shown in Fig. S4 b. Bar, 10 µm.