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. 2009 Aug 13;28(17):2554–2567. doi: 10.1038/emboj.2009.199

Figure 6.

Figure 6

The GLFG hydrogel allows facilitated entry not only of scImpβ but also of other nuclear transport receptors (NTRs). The entry of the six indicated GFP-NTR fusions (1 μM concentration on buffer side) into a saturated GLFG hydrogel was studied. Left panels show microscopic images in the GFP channel at the 30-s and 30-min time points, all taken at identical settings. Right panels show quantifications. Note that all tested NTRs entered the gel rapidly and showed a similar intra-gel movement, corresponding to a passage time through an NPC of ≈10 to 20 ms.