Table 2.
Escherichia coli expression vectors
| Name | Protein name | Expressed protein | Reference |
|---|---|---|---|
| pSF345 | Nsp1FG/FxFG | His10-TEV-Nsp1p2-601-Cys | Frey et al (2006) |
| pSF847 | Nup57GLFG-Nup49GLFG | His10-TEV-Nup57p1-233-Nup49p1-246-Cys | This study |
| pSF776 | Nup57GLFG-Nsp1FG/FxFG-Nup49GLFG | His10-TEV-Nup57p1-233-Nsp1p2-601- Nup49p1-246-Cys | This study |
| pSF851 | IBB-MBP-mEGFP | His14-TEV-IBB-MBP-mEGFP | This study |
| pSF844 | MBP-mCherry | His14-TEV-MBP-mCherry | This study; Shaner et al (2004) |
| pSF846 | mCherry | His14-TEV-mCherry | This study |
| pSF856 | IBB-MBP-mCherry | His14-TEV-IBB-MBP-mCherry | This study |
| pSF881 | IBB-ZsGreen | His14-TEV-IBB-ZsGreen | This study; Matz et al (1999) |
| pQE30-scImpβ | Importin β (Kap95p) | scImpβ-His6 | Görlich et al (1996b) |
| pSF582 | GFP-Pdr6p (Kap122p) | His10-GFP-TEV-Pdr6p | Frey and Görlich (2007) |
| pSF586 | GFP-Yrb4p (Kap123p) | His10-GFP-TEV-Yrb4p | Frey and Görlich (2007) |
| pSF587 | GFP-importin β (Kap95p) | His10-GFP-TEV-scImpβ | Frey and Görlich (2007) |
| pSF588 | GFP-Pse1p (Kap121p) | His10-GFP-TEV-Pse1p | Frey and Görlich (2007) |
| pSF879 | GFP-Crm1p | His10-GFP-TEV-Crm1p | This study |
| pQE60-hsTrn1 | Human transportin1 | His6-transportin1 | Izaurralde et al (1997) |
| pQE60-hsImpβ45-462 | hsImpβ45-462 | hsImpβ45-462-His6 | Kutay et al (1997b) |
| His6/His10/His14, histidine tag; IBB, importin β-binding domain (corresponding to amino acids 2-63 of Saccharomyces cerevisiae Srp1p); TEV, TEV protease recognition site. | |||