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. Author manuscript; available in PMC: 2009 Aug 18.
Published in final edited form as: Nat Protoc. 2008;3(10):1630–1638. doi: 10.1038/nprot.2008.150

Figure 3. Enrichment obtained after each step represented as the fraction of phosphopeptides over all the peptides identified.

Figure 3

(a) SCX chromatography alone enriches for phosphopeptides in early fractions. (b) Further IMAC enrichment over SCX fractions produces >75% phosphopeptides in most samples.