Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1983 Nov;18(5):1098–1101. doi: 10.1128/jcm.18.5.1098-1101.1983

Differentiation of staphylococci on the basis of nuclease properties.

R Gudding
PMCID: PMC272848  PMID: 6643663

Abstract

The quantity, thermostability, and serological pattern of nucleases produced by different staphylococci were studied. Staphylococcal strains were isolated from nine different species of animals or from humans. Staphylococcus aureus, Staphylococcus intermedius, and Staphylococcus hyicus subsp. hyicus were vigorous producers of nuclease, whereas the coagulase-negative staphylococci, except S. hyicus subsp. hyicus, produced significantly less nuclease. The nucleases of all strains were found to be thermostable. S. aureus, S. intermedius, and S. hyicus subsp. hyicus could be distinguished from each other and from coagulase-negative staphylococci on the basis of inhibition of nuclease activity by specific antibodies.

Full text

PDF
1098

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown R. W., Sandvik O., Scherer R. K., Rose D. L. Differentiation of strains of Staphylococcus epidermidis isolated from bovine udders. J Gen Microbiol. 1967 May;47(2):273–287. doi: 10.1099/00221287-47-2-273. [DOI] [PubMed] [Google Scholar]
  2. Devriese L. A., Hájek V. Identification of pathogenic staphylococci isolated from animals and foods derived from animals. J Appl Bacteriol. 1980 Aug;49(1):1–11. doi: 10.1111/j.1365-2672.1980.tb01038.x. [DOI] [PubMed] [Google Scholar]
  3. Devriese L. A., Oeding P. Characteristics of Staphylococcus aureus strains isolated from different animal species. Res Vet Sci. 1976 Nov;21(3):284–291. [PubMed] [Google Scholar]
  4. Erickson A., Deibel R. H. Production and heat stability of staphylococcal nuclease. Appl Microbiol. 1973 Mar;25(3):332–336. doi: 10.1128/am.25.3.332-336.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gudding R. DNases in milk and blood sera from different species. Acta Vet Scand. 1979;20(3):404–416. doi: 10.1186/BF03546602. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gudding R. Nuclease of staphylococcus epidermidis isolated from mastitic milk. Production and some properties. Acta Vet Scand. 1980;21(2):267–277. doi: 10.1186/BF03546889. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gudding R. The demonstration and characterization of deoxyribonucleases of streptococci group A, B, C, G and L. Acta Vet Scand. 1979;20(1):102–121. doi: 10.1186/BF03546634. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kloos W. E., Schleifer K. H. Simplified scheme for routine identification of human Staphylococcus species. J Clin Microbiol. 1975 Jan;1(1):82–88. doi: 10.1128/jcm.1.1.82-88.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kloos W. E., Wolfshohl J. F. Identification of Staphylococcus species with the API STAPH-IDENT system. J Clin Microbiol. 1982 Sep;16(3):509–516. doi: 10.1128/jcm.16.3.509-516.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lachica R. V., Genigeorgis C., Hoeprich P. D. Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity. Appl Microbiol. 1971 Apr;21(4):585–587. doi: 10.1128/am.21.4.585-587.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Phillips W. E., Jr, Kloos W. E. Identification of coagulase-positive Staphylococcus intermedius and Staphylococcus hyicus subsp. hyicus isolates from veterinary clinical specimens. J Clin Microbiol. 1981 Dec;14(6):671–673. doi: 10.1128/jcm.14.6.671-673.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sandvik O. The occurrence of antibodies against staphylococcal deoxyribonucleases in blood sera from different species. Acta Vet Scand. 1974;15(4):631–635. doi: 10.1186/BF03547232. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Scherer R. K., Brown R. W. Differentiation of staphylococcal and micrococcal proteinases by electrophoresis. Appl Microbiol. 1974 Nov;28(5):768–773. doi: 10.1128/am.28.5.768-773.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Victor R., Lachica F., Jang S. S., Hoeprich P. D. Thermonuclease seroinhibition test for distinguishing Staphylococcus aureus from other coagulase-positive staphylococci. J Clin Microbiol. 1979 Jan;9(1):141–143. doi: 10.1128/jcm.9.1.141-143.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES