Figure 4. Time course analysis of Spod-11-tox expression in challenged S. frugiperda larvae (E. coli 10⁵ bacteria/larvae).

A) Western blot analyses performed with protein extracts from hemocytes (from 6 to 72 hours post infection). B) Comparison of Spod-11-tox content in hemocytes and plasma early post infection. Proteins were loaded (10 µg/lane) on a Tris-Tricine 10–20% gradient gel. The western blot was realized as in figure 1. Numbers on the right sides of the blots indicate the molecular weight (kDa) estimated using Precision Plus Protein^TM Standards from Bio-Rad. Un; unchallenged.