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. 2009 Jul 30;10:57. doi: 10.1186/1471-2121-10-57

Figure 5.

Figure 5

Wnt signaling is involved in RA-promoted neural specification. A-C, Monolayer differentiation of 46C ESCs in RA-treated cultures (N2B27 + 10-8 M RA) exposed to the GSK-3 inhibitor LiCl (10 mM) or to NaCl (10 mM); A, Cultures of monolayer differentiation, shown in phase contrast or stained for Sox1GFP; B, Percentage of Sox1GFP+ cells scored by FACS. #, significant difference between inhibitor-treated cultures and NaCl-treated cultures (p < 0.001); C, Cultures of monolayer differentiation, shown in stained for Oct4 or combination of Oct4 and Hoechst; D-F, Monolayer differentiation of 46C ESCs in RA-treated cultures (N2B27 + 10-8 M RA) exposed to the GSK-3 inhibitor CHIR99021 (3 μM) or to equivalent amounts of DMSO diluents; D, Cultures of monolayer differentiation, shown in phase contrast or stained for Sox1GFP; E, Percentage of Sox1GFP+ cells scored by FACS; F, Cultures of monolayer differentiation, shown in stained for Oct4 or combination of Oct4 and Hoechst; G, Western blot analysis of Oct4 at day 5 under monolayer cultures; H, Q-PCR for Sfrp2 during monolayer differentiation. Scale bars: A, 40 μm; C, D, F, 80 μm.