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. 2009 Aug 27;4(8):e6757. doi: 10.1371/journal.pone.0006757

Figure 3. Subcellular distribution of BUBR1:GFP, BUB3.1:GFP and MAD2:GFP fusion proteins in propyzamid-treated tobacco cells.

Figure 3

Single optical section of prometaphase-like arrested cells expressing BUBR1:GFP, BUB3.1:GFP and MAD2:GFP fusion constructs, 1 h after propyzamid treatment. In merged images, the yellow colour corresponds to BUBR1:GFP, BUB3.1:GFP or MAD2:GFP (green channel) colocalisation with SYTO 82 (red channel). BUBR1:GFP, BUB3.1:GFP and MAD2:GFP localise in vivo to the kinetochores of chromosomes stained with SYTO 82 orange-fluorescent nucleic acid stain. Bars, 5 µm.