Table 2. Primers for qPCR analysis.
| Gene | GenBank | UniGene name | UniGene symbol | Forward primer | Reverse Primer | bp |
|---|---|---|---|---|---|---|
|
Hprt |
NM_012583 |
Hprt |
Rn.47 |
ATGGGAGGCCATCACATTGT |
ATGTAATCCAGCAGGTCAGCAA |
77 |
|
Ywhaz |
NM_013011 |
Ywhaz |
Rn.1292 |
CAAGCATACCAAGAAGCATTTGA |
GGGCCAGACCCAGTCTGA |
76 |
|
Rho |
NM_033441 |
Rho |
Rn.92530 |
GCAACAGGAGTCGGCTACCA |
GCATAGGGAAGCCAGCAGATC |
99 |
|
Tbp |
NM_001004198 |
Tbp |
Rn.22712 |
ACCAGAACAACAGCCTTCCACCTT |
TGGAGTAAGCCCTGTGCCGTAAG |
116 |
|
Ubc |
NM_017314 |
Ubc |
Rn.3761 |
TCGTACCTTTCTCACCACAGTATCTAG |
GAAAACTAAGACACCTCCCCATCA |
82 |
|
GluR1 |
NM_031608 |
Gria1 |
Rn.29971 |
GAGCAACGAAAGCCCTGTGA |
CCCTTGGGTGTCGCAATG |
80 |
|
GluR1 flip |
M38060 |
|
|
GAAGCAAGGACTCCGGAAGTAA |
GTAGAACACGCCTGCCACATT |
71 |
|
GluR1 flop |
M36418 |
|
|
GTCCGCCCTGAGAAATCCA |
AGCCCCTGCTCGTTCAGTT |
57 |
|
GluR2 |
NM_017261 |
Gria2 |
Rn.91361 |
AACGAGTACATCGAGCAGAGGAA |
GATGCCGTAGCCTTTGGAATC |
78 |
|
GluR2 short |
M38061 |
|
|
TTGAGTTCTGTTACAAGTCAAGGGC |
AGGAAGATGGGTTAATATTCTGTGGA |
81 |
|
GluR2 long |
NM_017261 |
|
|
GCCTTGGTTTGGCAATGC |
GACATCACTCAAGGTCATCTTCATTC |
92 |
|
GluR2 flip |
M38061 |
|
|
GGAACCCCAGTAAATCTTGCAGT |
GAGTCCTTGGCTCCACATTCAC |
107 |
|
GluR2 flop |
M36419 |
|
|
CATCGCCACACCTAAAGGATC |
CAATTTGTCCAACAGGCCTTGT |
88 |
|
GluR3 |
NM_032990 |
Gria3 |
Rn.74049 |
TTCGGAAGTCCAAGGGAAAGT |
CACGGCTTTCTCTGCTCAATG |
76 |
|
GluR3 flip |
M38062 |
|
|
GGAATGTGGAGCCAAGGACTC |
GCTCAGGCTTAGAGCACTGGTC |
58 |
|
GluR3 flop |
M36420 |
|
|
GGCAACCCCTAAAGGCTCAG |
AATACTGCCAGGTTAACAGCATTTC |
51 |
|
GluR4 |
NM_017263 |
Gria4 |
Rn.10938 |
GGCTCGTGTCCGCAAGTC |
TTCGCTGCTCAATGTATTCATTC |
77 |
|
GluR4 short |
S94371 |
|
|
TGATAGAGTTCTGTTACAAGTCCAGGG |
CGAGGAAGTTGGGTTAAAAGTCTGT |
86 |
|
GluR4 long |
NM_017263 |
|
|
CCAGGGCAGAGGCGAAG |
CGTTTTCTCCCACACTCCCA |
93 |
|
GluR4 flip |
M38063 |
|
|
TTTTGAAACTCAGTGAGGCAGG |
CGTACCACCATTTGTTTTTCAGC |
57 |
|
GluR4 flop |
M36421 |
|
|
CCTCTTGGACAAATTGAAAAACAA |
CCGCTGCCACATTCTCCTT |
57 |
|
GluR7 |
NM_181373 |
Grik3 |
Rn.92477 |
AAGGCAAAGGAGACCCGAAAG |
CATGGTTTCCCCGGTAGGTAAG |
110 |
|
NR1 |
NM_017010 |
Grin1 |
Rn.9840 |
CTGTTCTTCCGCTCAGGCTTT |
ATGAAGACCCCTGCCATGTTC |
200 |
|
NR2C |
NM_012575 |
Grin2c |
Rn.9709 |
GGATCTGCCAGAACGAGAAGA |
TTGTTGCCCCAGTTCTCGA |
390 |
| NR3A | AF073379 | Grin3a | Rn.42928 | CAGTCTTCGGAAACCTCATCG | TGACAGTTCTCATGCGCTTGT | 63 |
The primers used for qPCR were as shown. The GenBank accession codes and the anticipated size of the amplified product are listed.