Table 1. Oligonucleotides.
| Genotyping | ||
|---|---|---|
|
Gene, allele |
Forward |
Reverse |
|
Lif, wt |
AAATGCCACCTGTGCCATACGC |
CAACTTGGTCTTCTCTGTCCCG |
|
Lif, KO |
CTCTAAGCCTGAACTCTCTCATCC |
GATTCGCAGCGCAGCGCATCGCCTT |
|
Real-time PCR | ||
|
Gene |
Forward |
Reverse |
|
Edn2 |
AGACCTCCTCCGAAAGCTG |
CTGGCTGTAGCTGGCAAAG |
|
Ednrb |
ACCTACAAGTTGCTCGCAGAGG |
AAAACCTATGGCTTCGGGGAC |
|
Gfap |
CCACCAAACTGGCTGATGTCTAC |
TTCTCTCCAAATCCACACGAGC |
|
Fgf2 |
TGTGTCTATCAAGGGAGTGTGTGC |
ACCAACTGGAGTATTTCCGTGACCG |
|
Socs3 |
GGAGACAGATGAGGCTGGTGA |
GGACCTACTGACCGAGAGAT |
|
Stat3 |
CAAAACCCTCAAGAGCCAAGG |
TCACTCACAATGCTTCTCCGC |
|
Lif |
AATGCCACCTGTGCCATACG |
CAACTTGGTCTTCTCTGTCCCG |
| Clc | GCATCAACTCCGCAGCTTAG | CTGAACGCCATAGCCAGGTCT |
This table shows primers used for genotyping and real time PCR.