Table 1. PCR primers used in this study.
| Target | Primer | Primer sequence | Amplicon length | GenBank accession |
|---|---|---|---|---|
| K12 |
Forward |
ACTAGAGCCGACCTGGAAGC |
158 |
NM_010661 |
| Reverse |
ACCTTGGTGAGATCCACTCC |
|||
| K14 |
Forward |
ACCGCAAGGATGCTGAGGA* |
103 |
NM_016958 |
| Reverse |
GAAATCTCACTCTTGCCGCTCTG* |
|||
| DNp63 |
Forward |
CTGGAAAACAATGCCCAGACTCA |
126 |
AF075437
, AF075438, AF075439 |
| Reverse |
TGCGTGGTCTGTGTTGTAGG |
|||
| ß-actin | Forward |
CATCCGTAAAGACCTCTATGCCAAC* |
171 | NM_007393 |
| Reverse | ATGGAGCCACCGATCCACA* |
The asterisk indicates that the primer sequences for K14 and ß-actin were designed by Takara Bio Inc. (Otsu, Japan).