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Measurements of proteasome activity using a fluorescent peptide substrate. SH-SY5Y cells were transfected with pcDNA3 empty vector, wild-type α-synuclein (WT), A30P, A53T, S129D, Δ73-83 for 72 h or treated with 20 μM MG132 for 6 h. Cytosolic fractions were prepared and assayed using z-LLE-MCA as a substrate. The results were expressed as means + SD (n = 3).
