Figure 1. DNM3 gene expression increases during megakaryocyte development.
Cultures were initiated with purified human CD34+/CD38lo cells and maintained in media supplemented with IL3, IL6, SCF and Tpo. A) Fold increase in total nucleated cells at 0, 3, 5 10 and 14 days of culture. B) Percent of total cells expressing the CD41 antigen at indicated time points C) Relative fluorescent intensities from a single experiment for dynamins 1, 2 and 3 as detected on Affymetrix Gene Chip U133A \. Transcript levels for all three dynamins were determined from uncultured CD34+/CD38lo cells (Day 0) and their progeny cells harvested on days 5, 10 and 14 of culture in serum deprived media supplemented with IL3, IL6, SCF, and Tpo.