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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Pediatr Blood Cancer. 2008 Dec;51(6):754–759. doi: 10.1002/pbc.21727

Figure 2. The effect of MKP-1 knock down on cisplatin sensitivity in U2OS cells.

Figure 2

U2OS cells were transfected with MKP-1 siRNA. 48 and 72 hours after transfection, MKP-1 expression was determined by Western blot and cisplatin cytotoxicity measured by MTT assay. MKP-1 siRNA and NT represent MKP-1 siRNA and non-target transfected cells respectively; Control represents U2OS cells without transfection. A: Significantly decreased MKP-1 expression was observed at both 48 and 72 hours after transfection of MKP-1 siRNA. Actin was a loading control. B: Significantly increased cisplatin induced cell death was observed in MKP-1 siRNA transfected cells. At 48 hours after siRNA transfection, the cells were treated with 6.25 and 12.5 μg/ml of cisplatin for 24 hours.