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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Pediatr Blood Cancer. 2008 Dec;51(6):754–759. doi: 10.1002/pbc.21727

Figure 4. JNK activation and PRAR cleavage following cisplatin treatment.

Figure 4

P16T and U2OS cells were treated with cisplatin at 50μg/ml for 1, 2 & 4 hours. JNK phosphorylation (p-JNK) was undetectable in both P16T and U2OS cells prior to cisplatin treatment, but became evident at 2 hours after cisplatin treatment in P16T cells followed by PARP cleavage at 4 hours. In U2OS cells, p-JNK was not observed until 4 hours after cisplatin treatment and no PARP cleavage detected. MKP-1 expression was detected throughout the course in U2OS but not in P16T cells. p-JNK, cleaved PARP and MKP-1 were determined by Western blot. CTRL was a loading control.