Table 1.
Summary of IP target validation
| Predicted miR-184 target | IP enriched | IP miR-184 dependent | Up-regulated miR-184 depletion | UTR reporter assay* |
|---|---|---|---|---|
| sinu | + | + | + | +, K |
| pck | + | + | + | K |
| CG1298 | + | + | + | K |
| CG8121 | + | + | + | + |
| CG7713 | + | + | + | + |
| CG17218 | + | + | + | |
| CG2813 | + | + | + | |
| CG6583 | + | + | K | |
| CG9796 | + | + | + | |
| CG10620 | + | + | + | |
| CG13088 | + | − | + | + |
| CG1084 | + | − | + | K |
| CG6965 | + | − | − | |
| CG8010 | + | − | − | |
| CG3446 | + | − | − | |
| CG11059 | + | − | ||
| CG15154 | + | − |
Listed are the 17 genes with predicted miR-184 site that are enriched in the Ago1-IP microarray study with a minimum cutoff > 1.4 fold, P < 0.05. Seven out of 9 genes tested showed the dependence of IP enrichment on miR-184 (marked ″+″, blank = not tested). Twelve genes showed mRNA up-regulation upon miR-184 depletion (marked ″+″). Tested-positive and nonresponsive luciferase reporters in S2 cells are shown in column 5. *, K indicates UTR validation in ref. 7.