Abstract
Snell and Lapage proposed that the bacterium designated CDC group TM-1 be named Kingella denitrificans. Their description of the organism indicated that it produced acid from maltose which was in contrast with an earlier report from our laboratory that maltose was not utilized. In an attempt to explain the different findings in the two laboratories, the type strain and two additional strains of K. denitrificans were tested for acid production from maltose in phenol red broth, the rapid fermentation test, fermentation broth with Andrade's indicator, and oxidation-fermentation base. These media were tested without additional supplements and with supplements of horse serum (5% [vol/vol]) or rabbit serum (5% [vol/vol]). The results indicated that K. denitrificans does not produce acid from maltose. It was demonstrated that maltose in media containing serum is converted to glucose from which acid can be produced by K. denitrificans. The supplementation of maltose medium with horse serum by Snell and Lapage apparently had produced a false-positive maltose reaction. The description of K. denitrificans is emended to indicate that maltose is not utilized.
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Selected References
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- Brown W. J. Modification of the rapid fermentation test for Neisseria gonorrhoeae. Appl Microbiol. 1974 Jun;27(6):1027–1030. doi: 10.1128/am.27.6.1027-1030.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hollis D. G., Sottnek F. O., Brown W. J., Weaver R. E. Use of the rapid fermentation test in determining carbohydrate reactions of fastidious bacteria in clinical laboratories. J Clin Microbiol. 1980 Oct;12(4):620–623. doi: 10.1128/jcm.12.4.620-623.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hollis D. G., Wiggins G. L., Weaver R. E. An unclassified gram-negative rod isolated from the pharynx on Thayer-Martin medium (selective agar). Appl Microbiol. 1972 Nov;24(5):772–777. doi: 10.1128/am.24.5.772-777.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Riley P. S., Tatum H. W., Weaver R. E. Pseudomonas putrefaciens isolates from clinical specimens. Appl Microbiol. 1972 Nov;24(5):798–800. doi: 10.1128/am.24.5.798-800.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]