Table 1.

Experimental Measurements and Corresponding Global Fits to Binding Scheme^a

	DNA binding affinity (nM)			Metal binding affinity (mM)
Species	Apo E	EM2	EM4	Apo E	EM2
Experiments	KS	KS1	KS2	KM1	KM2
Gel shift assayb	n/a	n/a	0.11	n/a	n/a
Fluorescence anisotropyc	300±150	n/a	0.056±0.02	n/a	n/a
Nitrocellulose filter bindingc	n/a	n/a	0.053±0.01	n/a	n/a
ITCd	n/a	n/a	n/a	0.12±0.08	2.1±0.14
Global fit Trial 1	300c,e	10.6 (30%)	0.11b,e	0.088 (61%)	4.6 (11%)
Global fit Trial 2	300c,e	10.9 (17%)	0.22 (14%)	0.12d,e	2.1d,e

^aBinding scheme is illustrated in Fig. 3 and involves Ca(II) unless otherwise indicated.

^bRef. (37).

^cRef. (24).

^dRef. (27).

^eParameter is from an independent measurement and fixed. All other values and errors are derived from the global fit.