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. Author manuscript; available in PMC: 2009 Aug 18.
Published in final edited form as: Stem Cells. 2009 Mar;27(3):489–497. doi: 10.1634/stemcells.2008-0855

Fig.4. Constitutive expression of an oxygen-dependent degradation domain HIF-1α mutant triggers G6PT gene expression.

Fig.4

(A) Basal migration of MSC and MSC stably expressing a deletion mutant of HIF-1α (HIF-1α ΔODD, MSC-HIF) was performed as described in the Methods section. (B) Total RNA was extracted from MSC (white bars) and MSC-HIF (black bars), and qRT-PCR performed to assess the gene expression levels of G6PT, G6PC-3, VEGF, HIF-1α, and MT1-MMP. (C) Nuclear extracts were islolated from MSC and MSC-HIF and Westernblotting performed to detect nuclear HIF-1α or nuclear poly-(ADP-ribose) polymerase (PARP) expression. (D) Electrophoretic mobility assays were performed as described in the Methods section using nuclear extracts (Nuc. Extr.) isolated from MSC-HIF. NSC, cold unrelated non-specific competitor; SC, cold HIF-1α specific competitor; Ab, HIF-1α (1 µg) blocking antibody.