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. 2009 Sep;139(9):1619–1625. doi: 10.3945/jn.109.104638

FIGURE 2 .

FIGURE 2 

AMPK is involved in butyrate regulation of the barrier function in Caco-2 cell monolayers. (A) Caco-2 cells were incubated for 72 h in trans-well inserts with 2 mmol/L butyrate, 0.5 mmol/L AICAR, 10 μmol/L compound C (Cc), or butyrate with Cc and AICAR with Cc, respectively. (B) Extracts from Caco-2 cells at 72 h were immunoblotted for pAMPK, total AMPK, and actin. Lanes 1–6 are medium control, butyrate at 2 mmol/L, AICAR at 0.5 mmol/L, Cc at 10 μmol/L, butyrate+Cc, and AICAR+Cc, respectively. (C) Paracellular flux was determined using FITC-inulin probes in Caco-2 cell monolayers treated similarly for 72 h. In A and C, values are means + SE, n = 6. *Different from control, P < 0.05.