TABLE 3.
Monosaccharide analysis of peaks eluted from the LiChrospher NH2 column
| Contents of each monosaccharide in each peak (μmol/peak) | |||||
|---|---|---|---|---|---|
| Number of peaks | Fuc | Gal | Glc | GalNAc | GlcNAc |
| 6 | 0.045 | 0.074 | 0.002 | 0.005 | 0.065 |
| 7 | 0.055 | 0.063 | 0.025 | 0.005 | 0.050 |
| 8 | 0.062 | 0.051 | — | Trace | 0.031 |
| 9 | 0.016 | 0.030 | 0.004 | Trace | 0.011 |
| 11 | 0.020 | 0.041 | 0.008 | 0.005 | 0.043 |
Released monosaccharides were fractionated on a DEAE column and the neutral oligosaccharide pool was fractionated on the LiChrospher NH2 column. Some of the obtained peaks from the LiChrospher column were pooled and submitted to gas chromatographic analysis for their monosaccharide composition. Fuc, fucose; Gal, galactose; GalNAc, N-acetylgalactosamine; GlcNAc, N-acetylglucosamine.